X-ray crystallographic analyses of archaeal DNA binding proteins TrmBL2 and TrmB
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Histone and TK0471/TrmBL2 form a novel heterogeneous genome architecture in the hyperthermophilic archaeon Thermococcus kodakarensis
Being distinct from bacteria and eukaryotes, Archaea constitute a third domain of living things. The DNA replication, transcription, and translation machineries of Archaea are more similar to those of eukaryotes, whereas the genes involved in metabolic processes show more similarity to their bacterial counterparts. We report here that TK0471/TrmB-like 2 (TrmBL2), in addition to histone, is a no...
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In many hyperthermophilic archaea the DNA binding protein TrmBL2 or one of its homologues is abundantly expressed. TrmBL2 is thought to play a significant role in modulating the chromatin architecture in combination with the archaeal histone proteins and Alba. However, its precise physiological role is poorly understood. It has been previously shown that upon binding TrmBL2 covers double-strand...
متن کاملCrystal Structure of the Sugar Binding Domain of the Archaeal Transcritional Regulator TrmB
TrmB is an -glucoside-sensing transcriptional regulator controlling two operons encoding maltose/trehalose and maltodextrin ABC transporters ofPyrococcus furiosus. The crystal structure of an N-terminal truncated derivative of TrmB (amino acids 2–109 deleted; TrmB 2–109) was solved at 1.5 Å resolution. This protein has lost its DNA binding domain but has retained its sugar recognition site. The...
متن کاملStructural Insights into Nonspecific Binding of DNA by TrmBL2, an Archaeal Chromatin Protein.
The crystal structure of TrmBL2 from the archaeon Pyrococcus furiosus shows an association of two pseudosymmetric dimers. The dimers follow the prototypical design of known bacterial repressors with two helix-turn-helix (HTH) domains binding to successive major grooves of the DNA. However, in TrmBL2, the two dimers are arranged at a mutual displacement of approximately 2bp so that they associat...
متن کاملProtein–DNA binding dynamics predict transcriptional response to nutrients in archaea
Organisms across all three domains of life use gene regulatory networks (GRNs) to integrate varied stimuli into coherent transcriptional responses to environmental pressures. However, inferring GRN topology and regulatory causality remains a central challenge in systems biology. Previous work characterized TrmB as a global metabolic transcription factor in archaeal extremophiles. However, it re...
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تاریخ انتشار 2015